It's a picture of Samberg with Kevin James, Will Ferrell, and Adam Sandler from the MTV movie awards in 2007.
Some think Samberg doesn't look excited enough in this pic (as he should be), but I think he'll just learn to smile more like the other dudes are.
Bonus pics from the 2007 MTV Movie Awards...
With Shia LaBeouf:
With Sarah Silverman: